Hi guys, Welcome to the Advanced Video Search Engine, and you are watching Gene Transfer Methods /Techniques in Plants #biotechnologyanditsapplications direct &indirect method. That video is uploaded by Biology by Dr. Imtiyaz at 2020-05-19T23:10:54-07:00. We are promoting this video only for entertainment and educational purpose only. So, I hope you like & enjoy it. [D5aEKiez_nw]
| Name | Gene Transfer Methods /Techniques in Plants #biotechnologyanditsapplications direct &indirect method |
| Video Uploader | Video From Biology by Dr. Imtiyaz |
| Upload Date | This Video Uploaded At 20-05-2020 06:10:54 |
| Video Discription | Join my telegram group to get the pdf notes: https://t.me/BiologyByImtiyaz For pdf notes and Book visit https://www.biologylectures.com/ It is a technique to efficiently and stably introduce foreign genes (Gene of interest) into the genome of target cells or transferring a gene into a non native organism and the process is called Transgenesis. There are two methods of gene transfer in plants. 1. Direct or Vector-less Gene Transfer. a. Physical i. Particle gun ii. Electroporation iii. Microinjection b. Chemical Method i. Liposome ii. PEG 2. Indirect or Vector-Mediated Gene Transfer a. A.tumefaciens (produces a gall) b. A. rhizogenes (produces excessive root hairs) #biotechnology #recombinantdnatechnology #bscbotany #12biologyclass #11biology #biotechnologyanditsapplications #6thsemester #12thbiology Particle gun The foreign DNA containing gene of interest is coated onto beads/surface of either Au or tungsten (1-um) called as micro carrier. The coated beads are then attached to the end of the plastic bullet (Macro carrier) and loaded into the firing chamber of the gene gun and then bombarded on target cells by particle gun. This method avoids the need of protoplast and is better in efficiency. This technique can be used for any plant cells, root section, embryos, seeds and pollen or even on intact plant in the experimental filed. Though some cells get destroyed but on the other side many cell receive foreign DNA and thus can produce large no of transforming plant cell. Biolistics technique has been used successfully to transform soyabean, cotton, spruce, sugarcane, papaya, sunflower, rice, maize, wheat, tobacco etc Electroporation Introduction of DNA into plant cell by exposing them for very brief periods into high voltage electric pulses, which induce transient pores in the plasma membrane, is called electroporation. If plant cell (Protoplast) is placed in medium containing GoI then the DNA enter into plant cell via pores and finally incorporated into plant genome. Advantage: Some plants are PEG sensitive so electroporation may be of choice, method is fast, less costly and high percentage of stable transformants can be produced. It has been used to produce stably transformed cell lines and plants of several species like tobacco, maize, rice, wheat, etc. D5aEKiez_nw |
| Category | Education |
| Tags | Education Download MP4 | Education Download MP3 | Education Download MP4 360p | Education Download MP4 480p | Education Download MP4 720p | Education Download MP4 1080p |
